Double embryo transfer of cows, transferring double embryos to cows with uterine pregnancy changes after synchronous estrus treatment. And use animal ultrasound to examine the uterus, and transfer two qualified embryos at once on the corpus luteum side of the qualified recipient cow during transplantation, thus enabling the cow to produce twins.

Two embryos were transferred to the recipient cattle and early pregnancy examination was performed using Veterinary Ultrasound. The success rate of twin pregnancy was 57.1%, and it was found that some embryos that had moved to the same side of the corpus luteum uterine horn automatically migrated to the opposite side. Two IVF frozen embryos were transferred into each recipient cow, and early pregnancy examination was performed using veterinary ultrasound. The success rate of twin pregnancy was 25%.

Due to the influence of the success rate of transplantation, transferring two embryos at once, if neither of them becomes pregnant, the cost is too high. If pregnant, through animal B-ultrasound examination, heterosexual twin infertility may also occur. Therefore, it is generally suitable for improving embryo utilization and twin rate during fresh embryo transfer to accelerate the breeding of high-quality cattle.

After embryo segmentation, twin pregnancy is achieved by transferring a well developed 7-day-old embryo into two equally sized halves using a microscope or by hand. The two halves are then placed in tubes and transferred into a qualified recipient's deep corpus luteum uterine horn. The method involves early pregnancy examination using animal B-ultrasound to determine if the embryo is a twin.

By using embryo segmentation to expand the source of embryos, inducing monozygotic twin calves can also achieve a higher twin rate and solve the problem of infertility in opposite sex twin female calves. Due to the absence of infertility caused by opposite sex twins, the success rate and economic benefits of transferring two half embryos after embryo division to produce twin calves are high. But the required technical level is high, the related instruments are precise, the cost is high, and it is difficult to promote and apply.

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