The use of OPU live egg collection instrument for cow live egg collection technology provides an opportunity for the application of in vitro embryo production technology in animal breeding. The donor cows that provide high-quality oocytes can be efficiently reused, and the embryos obtained from in vitro fertilization have a clear lineage, thus having seed value.

1. The OPU live egg retrieval device has high efficiency in embryo production

From each cow, 10-20 oocytes can be collected per week, with a blastocyst development rate of 30%, resulting in an average of 3-6 usable embryos per cow. The conception rate of fresh embryo transfer can reach 40%, and the conception rate of frozen embryo transfer can also reach 35%. According to this ratio, each cow can obtain dozens of IVF calves per year. It can produce a large number of high-quality and affordable embryos, solving the problem of embryo source for the promotion of embryo transfer technology.

2. Wide range of applications

The application of live egg harvesting technology can continuously supply eggs to heifers with excellent genetic potential, low reproductive age, poor superovulation effect, high-yielding cows with reproductive diseases, aging or pregnancy, and high-quality beef cattle. Combined with related biotechnology such as in vitro embryo production, it can shorten the breeding cycle, fully tap the breeding potential of high-quality cattle, and mass produce high-quality embryos. The OPU live egg retrieval device can enable excellent cows without reproductive ability due to reproductive diseases to obtain offspring; A large number of antral follicles can be developed and utilized to fully unleash the reproductive potential of excellent cows; It can enable immature calves to reproduce offspring, greatly shortening the generation interval in breeding.

3. Low production cost with ease

With the popularization and promotion of embryo engineering technology, the application prospects of live egg collection in vitro embryo production technology are broad. Using traditional natural breeding methods, one cow only produces one offspring per year; The OPU live egg retrieval device utilizes superovulation embryo transfer technology, allowing one cow to obtain 20-30 embryos and 10-15 offspring per year; By using live egg collection and in vitro embryo production technology combined with embryo transfer technology, one cow can obtain more than 100 embryos and dozens of offspring per year. If combined with genetic control technology, the desired offspring can be obtained, and the results are obvious. With the expansion of high-quality livestock, its potential economic and social benefits become more apparent.

4. Easy to produce and control embryos

The OPU-IVP technology can be combined with sperm separation technology to produce pre predicted embryos, achieving the goal of controlling other types of embryos. Due to the current low efficiency of sperm separation, each instrument can separate 100-200 small tubes with a total sperm count of 1 million per day, which is much lower than the standard of 10 million effective sperm per tube for conventional breeding; Moreover, semen is expensive, with a market price of about 400-500 yuan per sperm, and the fertilization rate of separated sperm is low. It is not practical to use it as a routine artificial insemination. As a routine superovulation embryo production method, it will greatly increase the cost of embryos. However, when combined with in vitro embryo production technology, this problem can be solved by using sex controlled semen for mass production of low-cost sex controlled embryos.

5. It can provide rich experimental materials and necessary technical platforms and auxiliary means for nuclear transfer and gene transfer, accelerate the progress of basic research, and deepen the understanding of theoretical issues such as the occurrence and maturation of oocytes, sperm capacitation and fertilization, and early embryo development through the OPU live egg collection instrument.

Of course, we must also recognize that this technology involves multiple steps, high technical content, and requires high professional operation skills from technicians. The investment is also higher than the cost of producing embryos in vivo. However, this technology has significant scientific significance and application value for the development of China's cattle industry, as well as for the research of developmental biology and other embryonic biotechnology. The OPU live egg collection device technology can not only be efficiently used for breeding cows and beef cattle in cattle production units, establishing a breeding system for high-quality cattle, but also for research in embryonic biotechnology and developmental biology.

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